antiproliferating cell nuclear antigen (pcna) pc10  (Agilent technologies)

Journal: Oxidative Medicine and Cellular Longevity

Article Title: Apoptosis in the Mammary Gland of Virgin Rats Subchronically Fed With a Vitamin A Deficient Diet

doi: 10.1155/omcl/6334165

Figure Lengend Snippet: VAD and further refeeding with a VAS diet cause changes in cell proliferation in the mammary gland. (A) Immunohistochemistry for PCNA in the mammary gland. The black arrows indicate positive PCNA + cells. Magnification 100×. The scale bar represents 25 μm. (B) Quantification of the percentage of PCNA + cells from the immunohistochemistry images shown in (A). Data are shown as representative images or mean values ± SDs ( n = 10).

Article Snippet: The sections were incubated with the following primary antibodies: 8 h in a humidified chamber at 4°C with mouse monoclonal antihuman BCL-2 (clon BCL-2/100; Catalog. No. AM287-5M, BioGenex, San Ramón, CA, USA), 30 min in a humidified chamber at 20°C with rabbit polyclonal antihuman BAX protein (Catalog. No. AR347-5R, BioGenex, San Ramón, CA, USA), and for 4 h in a humidified chamber at 20°C with mouse monoclonal anti-rat proliferating cell nuclear antigen (PCNA) (clon PC10; Catalog. No. AM252-5M, BioGenex, San Ramón, CA, USA).

Techniques: Immunohistochemistry

Journal: Oxidative Medicine and Cellular Longevity

Article Title: Apoptosis in the Mammary Gland of Virgin Rats Subchronically Fed With a Vitamin A Deficient Diet

doi: 10.1155/omcl/6334165

Figure Lengend Snippet: VAS and further refeeding with a VAS diet cause changes in apoptosis and proliferation in the mammary gland. Determination of the TUNEL + /PCNA + cell ratios from the immunohistochemistry images shown in Figures A and A. The data are presented as the means ± SDs ( n = 4).

Article Snippet: The sections were incubated with the following primary antibodies: 8 h in a humidified chamber at 4°C with mouse monoclonal antihuman BCL-2 (clon BCL-2/100; Catalog. No. AM287-5M, BioGenex, San Ramón, CA, USA), 30 min in a humidified chamber at 20°C with rabbit polyclonal antihuman BAX protein (Catalog. No. AR347-5R, BioGenex, San Ramón, CA, USA), and for 4 h in a humidified chamber at 20°C with mouse monoclonal anti-rat proliferating cell nuclear antigen (PCNA) (clon PC10; Catalog. No. AM252-5M, BioGenex, San Ramón, CA, USA).

Techniques: TUNEL Assay, Immunohistochemistry

Journal: Life

Article Title: Anti-Cataract Effect of the Traditional Aqueous Extract of Yerba Mate ( Ilex paraguariensis A. St.-Hil.): An In Ovo Perspective

doi: 10.3390/life14080994

Figure Lengend Snippet: Effect of YM on PCNA ( A ) and Caspase-3 H ( B ) in lenses. Data are expressed as the means ± SD, a p < 0.05 vs. C, b p < 0.05 vs. HC. C: control group, HC: hydrocortisone group, YM: yerba mate aqueous extract, YM-1: YM 62.5 mg/kg group, YM-2: YM 125 mg/kg group, YM-3: YM 250 mg/kg group.

Article Snippet: The prepared samples were stained with antiproliferative cell nuclear antigen (PCNA) (Novus, NB500-106, Littleton, CO, USA) to assess proliferation and caspase-3 (Novus-NB100-56708) to assess apoptosis (antibodies are compatible with chick).

Techniques: Control

Journal: Life

Article Title: Anti-Cataract Effect of the Traditional Aqueous Extract of Yerba Mate ( Ilex paraguariensis A. St.-Hil.): An In Ovo Perspective

doi: 10.3390/life14080994

Figure Lengend Snippet: Caspase-3 ( a ) and PCNA ( b ) staining in lenses. I: C, II: HC, III: YM-1, IV: YM-2, V: YM-3. C: control group, HC: hydrocortisone group, YM: yerba mate aqueous extract, YM-1: YM 62.5 mg/kg group, YM-2: YM 125 mg/kg group, YM-3: YM 250 mg/kg group.

Article Snippet: The prepared samples were stained with antiproliferative cell nuclear antigen (PCNA) (Novus, NB500-106, Littleton, CO, USA) to assess proliferation and caspase-3 (Novus-NB100-56708) to assess apoptosis (antibodies are compatible with chick).

Techniques: Staining, Control